Figure 7.

GST-pulldown assay carried out with C. jejuni total protein extracts. Immunoblot analysis of samples obtained from the GST-pulldown assay performed with C. jejuni total protein extract, stained with an anti-GroEL antibody. Samples recovered from the column containing either GST alone or GST-HrcA-Glutathione-agarose slurry (lanes labeled “PD”) were separated by SDS-PAGE and transferred on a PVDF membrane, together with samples that passed through the column, without interacting with baits (i.e., flowthrough samples, labeled “FT”). In addition, an aliquot of the purified GroEL protein was included as positive control (lane labeled “GroEL”). The intensities of the GroEL-specific bands in the GST- and GST-HrcA-PD samples were quantified using the ImageQuant software: the GroEL band in the GST-HrcA PD sample is 6.2-fold more intense than the same band in the GST PD control sample. The positions of the molecular mass standards are shown on the left. Symbols: : × and ×× indicate cross-reactive bands.