Figure 2.

Effect of ORLE on rat macrophages. (A) CD-68 expression (positive cells/mm²) in colon tumours from PIRC rats fed with a control diet (black column) or a diet enriched in ORLE (green column). (B) iNOS expression in colon tumours (T) from PIRC rats fed with a control diet (black columns) or a diet enriched in ORLE (green columns) compared to iNOS expression in normal mucosa (NM). Data are expressed as means + SEM; two-way-ANOVA of the data shows a statistical significance (** p = 0.0016) for the effect of tissue (i.e., tumour expression being higher than that in the normal mucosa), while dietary treatment was not significant. Post-hoc analysis of the differences between different groups (Tukey’s multiple comparisons test) shows a significant difference between NM and tumours in the Control diet (** p = 0.0047), while the difference between NM and tumours in the ORLE diet is not significant. (C) Effect of ORLE on NO production by peritoneal macrophages recovered from tumour bearing rats fed a control diet (black columns) or a diet enriched in ORLE (green columns) and untreated or exposed, in vitro, to IFNγ/LPS. Two-way-ANOVA of the data shows a statistical significance for the effect of ORLE diet (p = 0.0001), and for the effect of treatment (p = 0.0002). Post-hoc analysis of the differences between different groups (Tukey’s) shows a significant difference as indicated by the asterisks (p < 0.001).