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. 2021 Oct 6;10(10):1577. doi: 10.3390/antiox10101577

Figure 3.

Figure 3

MTT assay of HTC116 cells exposed for 72 h to different concentration of ORLE (A). Data are expressed as means ± SEM of the percentage of viability and are representative of three independent experiments (n = 4). Statistical evaluation of the effect of ORLE on tumour cell viability was analyzed by one-way ANOVA with post-hoc Tukey’s test, * p < 0.001, ** p < 0.0001, and *** p < 0.00001. (B) Inhibition of intracellular protein expression of iNOS in HCT116 cells. Representative histogram plots of untreated HCT116 cells (blu istogram) or HCT116 cells exposed for 72 h to ORLE (50 μM) (green istograms). Coloured istograms represents HCT116 cells exposed to primary and secondary antibody, while white istograms represents cells exposed to secondary antibody only. (C) Quantitative analysis of mean florescence intensity. Data were expressed as means ± SEM (n = 3). Statistical significance was determined using two-tailed unpaired student’s t-test corresponding to * p < 0.0001.