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. 2021 Sep 29;11(10):1429. doi: 10.3390/biom11101429

Figure 5.

Figure 5

N-Acetylcistein decreases senescence-associated β-Gal assay in MAEC treated with BPA 5 days. (A) Senescence-associated β-Gal assay in MAEC treated with BPA 5 days at 5 µM and BPA 5 µM + NAC 5 mM. Note that pretreatment with NAC in BPA-treated cells reduced the percentage of senescent cells even below the control group. Results represented are means ± SD. (n = 4, each performed in triplicate). **** p < 0.0001 using Kruskal–Wallis test. (B) Representative microphotographs of the senescence assay made at x40 magnification. (C) Western blot analysis of MAEC treated as in A, using antibodies to p21 and p16. Data are the means ± SD of four different experiments, each performed in duplicate. * p < 0.05 using Kruskal–Wallis test for the comparison between control and BPA-treated cells; ** p < 0.001 using Kruskal–Wallis test for the comparison between BPA 5 µM and BPA 5 µM + NAC 5 mM.