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. 2021 Sep 29;9(10):1349. doi: 10.3390/biomedicines9101349

Figure 3.

Figure 3

A549 cell lysate induced IL2 expression to stimulate GZMB and PRF1 overexpression in CD8+ T cells. (A) Healthy CD8+ T cells were incubated with parental and irradiated A549 cells for 24 h, and which were isolated consequently and analyzed for expression of CD8+ T cells markers using qPCR, including the genes associated with the activation: GZMB, PRF1, IFNγ, CD122 (IL2RB), inhibition: CD279 (PD-1), transcriptional factor: EOMES, FLI1, TCF1, RUNX3, and migration receptor: CXCR3. (B) CD8+ T cells were individually treated with A549 cell lysate or 20ng/mL of IFNα for 2 h, and IL2 expression was detected using qPCR. (C) CD8+ T cells were treated with 20 ng/mL of IL2 for 2 h, and the genes of CD8+ T cell markers were detected using qPCR. The quantification was calculated based on 3-times repeat in qPCR assay. * p < 0.05.