Table 1.
Observations supporting either the Pib2-EGO parallel or cooperative model.
| Observation | Supported Model |
Yeast Strain Background | Culture Media/ TORC1 Stimuli |
TORC1 Readout |
References |
|---|---|---|---|---|---|
|
pib2∆-EGO∆ synthetic lethality |
Parallel | BY4741/4742 (auxotroph) |
YPD | Viability (rescued by hyperactive TORC1) |
[12] |
| Pib2-EGO redundancy in the basal TORC1 activity |
Parallel | BY4741 (auxotroph) |
SD with casamino acid |
Sch9 phosphorylation (C-terminus band-shift) |
[38] |
| Partial defects in TORC1 activation (>10 min) in pib2∆ and EGO∆ |
Parallel | Σ1278b (prototroph) [32]; S288C (prototroph) [36] |
Minimal proline medium +glutamine or ammonium stimulation [32]; SD-N +glutamine stimulation [36] |
Rps6 phosphorylation (phospho-specific antibody) or Npr1 phosphorylation (band-shift) [32]; Sch9 phosphorylation (phospho-specific antibody) [36] |
[32,36] |
| Distinct Pib2-TORC1 and EGO-TORC1 supercomplexes (co-immunoprecipitation) |
Parallel | BY4741 (auxotroph) |
YPD | N/A | [38] |
| Pib2-EGO interaction (yeast two-hybrid) |
Cooperative | N/A | N/A | N/A | [12,49] |
| Strict requirement of both Pib2 and EGO for acute and transient TORC1 activation (<5 min) |
Cooperative | BY4741/4742 (prototroph) [35]; S288C (prototroph) [36]; W303A (auxotroph) [39] |
Minimal proline medium +glutamine stimulation [35]; SD-N +glutamine or arginine stimulation [36]; SD-N +glutamine or leucine stimulation [39] |
Sch9 phosphorylation (phospho-specific antibody) [35,36]; Rps6 phosphorylation (phospho-specific antibody [39] |
[35,36,39] |