Figure 5.
The combination of polyphenols induces elevation of cytosolic calcium levels in prostate cancer cells. Fluo-4 emission was recorded kinetically at 1 min intervals, starting immediately upon the addition of 7 μM CUR, 5 μM CA or their combination (CUR + CA). The bars show peak [Ca2+]cyt signals recorded at 6–8 min (as in Supplementary Figure S3) relative to DMSO-treated cells. Data are presented as mean ± SEM (n = 3). Statistically significant differences for CUR, CA or CUR + CA vs. DMSO (** p < 0.01 and *** p < 0.001) and for CUR and CA vs. CUR + CA (# p < 0.05; ## p < 0.01 and ### p < 0.001). § p < 0.05 and §§ p < 0.01, significant difference between the two indicated groups (Student’s t-test).