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. 2021 Sep 27;10(10):967. doi: 10.3390/biology10100967

Figure 3.

Figure 3

HCQ (60 μM) and CPZ (0.1 mM) attenuated acute INF-induced the increase of apoptosis level, caspase activates via the increases of cell viability in the U937 cell (Mean ± SD and n = 6). The column bars of (A–F) are indicating the changes of apoptosis, cell viability, CAS3, CAS9, ΔΨm, and cROS in the six groups. For the apoptosis analyses, a commercial kit (Biocolor APOPercentage) was used, although cell viability level was analyzed by using MTT. The Ac-DEVD-AMC and Ac-LEHD-AF fluorogenic substrates were used for the analyses of cleavage CAS3 and CAS9, respectively. For the determination of ΔΨm, and cROS, the stains of JC1 and DCFH-DA were used (* p ≤ 0.05 vs. Cntr, HCQ, and CPZ. ** p ≤ 0.05 vs. INF). The significance of p-values was analyzed by using the test of Kruskal–Wallis.