Skip to main content
. 2021 Oct 8;10(10):1583. doi: 10.3390/antiox10101583

Figure 2.

Figure 2

Alteration of mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) generation by β-sitosterol in the two cell types. (A,B) We used 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide (JC-1) dye to investigate the alteration of MMP by β-sitosterol (0, 10, 25, and 50 µg/mL). Quadrants of the dot blot represent the state of MMP in ES2 and OV90 cells. Comparative bar graphs represent the loss of MMP compared to the control group (100%) in ES2 and OV90 cells. (C,D) DCF fluorescence intensity was analyzed to investigate the change in ROS production following β-sitosterol treatment (0, 10, 25, and 50 µg/mL). The histograms represent the state of ROS production in ES2 and OV90 cells. The comparative bar graphs represent ROS production compared to the vehicle-treated control (100%) in ES2 and OV90 cells. DCF: dichlorofluorescein. (E) Alteration of MMP by β-sitosterol with or without NAC (1 mM) was determined. The asterisks indicate significant differences between treated and control cells (*** p < 0.001, ** p < 0.01, and * p < 0.05); # indicates significant differences as compared to cells treated with β-sitosterol alone.