WISP-3 promotes VEGF-C-dependent lymphangiogenesis in human chondrosarcoma. (A,B) Cells were incubated with WISP-3 (10–100 ng/mL) and levels of VEGF-C mRNA and protein expression were examined by qPCR and ELISA assays. (C,D) Collected conditioned medium (CM) was applied to lymphatic endothelial cells (LECs), then LEC tube formation was measured (scale bar = 100 μm). Quantitative results are expressed as the mean ± SD. All experiments were repeated 3 to 5 times (solid squares, solid dots, hollow circles and solid triangles). * p < 0.05 compared with the control group; # p < 0.05 compared with the WISP-3-treated group.