Figure 5.

WISP-3 facilitates VEGF-C synthesis by inhibiting miR-196a-5p. (A) JJ012 cells were incubated with WISP-3. MiRNA expression was determined by the qPCR assay. (B,C) Cells were incubated with WISP-3. MiR-196a-5p or pre-miR-196a-5p expression was determined by the qPCR assay. (D) Cells were transfected with miR-196a-5p mimic, then stimulated with WISP-3. VEGF-C levels were determined by qPCR. (E,F) Cells were treated with the indicated inhibitors or siRNAs, then stimulated with WISP-3. MiR-196a-5p expression was quantified by qPCR. Quantitative results are expressed as the mean ± SD. All experiments were repeated 3 to 5 times (solid squares, solid dots, hollow circles and solid triangles). * p < 0.05 compared with the control group; # p < 0.05 compared with the WISP-3-treated group.