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. 2021 Sep 28;9(10):1343. doi: 10.3390/biomedicines9101343

Figure 3.

Figure 3

Modulation of LMNA gene expression in GBM T98G cells. (A) Relative quantification (RQ) of LMNA gene in LMNA-KD (dashed) and LMNA+ overexpressed (black) T98G cells as analysed by qRT-PCR. The data are reported as the level of mRNA relative to the respective transfected vector control cells and are the means + SD (n = 3). Statistical significance: LMNA+ and KD vs mock **** p < 0.0001. (B) Top, representative western blot of the Lamin A/C protein in LMNA-KD (KD) and LMNA+ T98G cells; bottom, blots densitometry as analyzed by ImageJ software. Values are averages + s.d. (n = 3), relative to mock cells (white) of three independent experiments with similar results. LMNA-KD (dashed), LMNA+ (black). GAPDH was used as loading control.). Statistical significance: LMNA+ and KD vs mock ** p < 0.01. (C) Representative confocal images of mock, LMNA-KD (KD) and LMNA+ cells. Red, Lamin A/C immunostaining; blue, DAPI. Scale bar: 10 microns.