Figure 11.

Effect of TMF on TNF–α–induced phosphorylation and translocation of NF-κB in HDFs. (A) Expression and phosphorylation of NF–κB (p65) was determined by immunoblotting. The data are expressed as mean ± SEM (n = 3). # p < 0.05 compared to non-treated group and * p < 0.05 compared to TNF-α-treated group. (B) Translocation of NF-κB (p65) from the cytoplasm to nuclear was visualized using immunofluorescence staining (40× magnification, 100 µm scale bar). Abbreviations: TMF, 5,7,4′-trimethoxyflavone, and TNF-α, tumor necrosis factor alpha.