Figure 8.

Impact of the single protein surfacing on nanoparticle cell uptake in hMSCs. (A) The polystyrene nanoparticle (carboxy functionalized, PSCOOH) was coated with ApoA4, ApoC3, AntIII, prothrombin, or ApoH (50 μg per 0.05 m², 1 h, 37 °C), and incubated for 6 h in a serum-free medium. Error bars ± SEM estimated with two experiments in triplicate (n = 3). Level of significance (* p < 0.05; ** p < 0.01). (B) Accompanying confocal microscopy images of living hMSCs treated with ApoA4 or ApoH-coated carboxy functionalized polystyrene nanoparticle. Plasma membrane stained with cell mask orange (red) and nanoparticles labeled with Bodipy-1. Scale bars = 75 μm. Adapted, with permission, from [62].