Figure 3.

GYY-mitigated elevated expression of RXRα, RXRβ and RARγ1 in diabetic kidney and MCs under HG condition. Protein was extracted from (A) saline- or GYY-treated kidneys from WT and Akita mice and (B) untreated or GYY-treated mouse MCs in NG or HG condition and analyzed for the expression of RXRα, RXRβ and RARγ1 by Western blot. The expression of each protein was normalized with GAPDH. The bar graphs represent the mean fold change ± SD vs. WT + Saline or NG. n = 6/group or 3 independent experiments, * p  <  0.05 vs. WT + Saline or NG, ^† p  <  0.05 vs. Akita + Saline or HG, ^# p <  0.05 vs. WT + GYY.