Figure 4.

Upregulated PAI-1 expression was normalized in diabetic kidney and MCs under HG condition by GYY treatment. Protein was extracted from (A) saline- or GYY-treated kidneys from WT and Akita mice and (B) untreated or GYY-treated mouse MCs in NG or HG condition and analyzed for PAI-1 expression by Western blot. The expression of PAI-1 was normalized with GAPDH. The bar graphs represent the mean fold change ± SD vs. WT + Saline or NG. n = 6/group or 3 independent experiments, * p  <  0.05 vs. WT + Saline or NG, ^† p  <  0.05 vs. Akita + Saline or HG.