Figure 5.

Elevated expressions of MMP-9 and MMP-13 were alleviated by GYY treatment in diabetic kidney. (A) Saline- or GYY-treated kidneys from WT and Akita mice were collected in Trizol for total RNA extraction, and semi-quantitative RT-PCR analyses were performed for MMP-9 and MMP-13 gene expression. (B) Protein was extracted from the saline- or GYY-treated kidneys from WT and Akita mice and analyzed for the expression of MMP-9 and MMP-13 by Western blot. GAPDH was used as a loading control for all RT-PCR and immunoblot analyses. The bar graphs represent the mean fold change ± SD vs. WT + Saline. n = 6/group, * p  <  0.05 vs. WT + Saline, ^† p  <  0.05 vs. Akita + Saline.