Skip to main content
. 2021 Oct 7;11(10):1477. doi: 10.3390/biom11101477

Figure 6.

Figure 6

Elevated expressions of MMP-9 and MMP-13 were alleviated by GYY treatment in MCs under HG condition. (A) Untreated or GYY-treated mouse MCs in NG or HG condition were collected in Trizol for total RNA extraction, and semi-quantitative RT-PCR analyses were performed for MMP-9 and MMP-13 gene expression. (B) Protein was extracted from the untreated or GYY-treated mouse MCs in NG or HG condition and analyzed for the expression of MMP-9 and MMP-13 by Western blot. GAPDH was used as a loading control for all RT-PCR and immunoblot analyses. The bar graphs represent the mean fold change ± SD vs. NG. n = 3 independent experiments, * p  <  0.05 vs. NG, † p  <  0.05 vs. HG.