Figure 10.

GYY mitigated the increased expression of fibronectin and laminin in diabetic kidney. Representative photomicrograph of kidneys immunostained with specific primary and fluorescent-tagged secondary antibodies against (A) fibronectin (red, white arrow) and (B) laminin (green, yellow arrow). The nuclei were counterstained with DAPI (blue). The bar graphs represent the mean fold change ± SD in fluorescence intensity vs. WT + Saline for (C) fibronectin and (D) laminin. n = 6/group, * p  <  0.05 vs. WT + Saline, ^† p  <  0.05 vs. Akita + Saline. Magnification, × 60; scale bar, 20 µm.