Figure 2.

Experimental overview|Two S. bicolor genotypes (SSG 59-3 and PC-5) with varied salt tolerance were phenotypically evaluated based on germination studies and then planted in screen house under different salt concentrations. Leaves from 5 plants were pooled and considered a biological replicate, and two such replicates were used in the analysis. Total RNA was isolated from sorghum leaves; sequencing libraries were generated. After library construction, they were sequenced on an Illumina Hiseq-2500 platform to obtain 125 bp/150 bp paired-end reads. The raw data generated were processed and differential expression analysis was performed, followed by phylogenetic analysis, as well as analyses of protein characteristics and gene structure.