Table 3.
NR-PKS clusters detected in both chemotypes of P. furfuracea, the PKS domains and other genes present in the cluster.
| Cluster Number | PKS | Compound Defining the Group (Kim et al. [11]) | PKS Category (Kim et al. [11]) | PKS Domains | Total Genes in the Cluster | Identified Genes in Cluster | Most Similar Known PKS (MIBiG) | Coverage and Similarity to MIBiG Cluster |
| 1 | Pfur12-3_001319 | VII (azaphilones, monascorubrin) | not known | SAT-KS-AT-ACP cMT-TD | 12 | Regulatory gene, NRPKS | monascorubrin | 95%, 58% |
| 2 | Pfur18-1_002705 | V (anthraquinones) | PKS20 | SAT-KS-AT-PT-ACP | 16 | metallo-beta-lactamase family protein, NRPKS, halogenase | RES-1214-2 | 61%, 100% |
| 3 | Pfur44-1_008033 | II (melanins) | PKS15 | SAT-KS-AT-PT-ACP ACP-TE | 11 | PKS, dehydrogenase/reductase (KR) | naphthalene | 99.5%, 53% |
| 4 | Pfur33-1_006185 | I (zearalenone, orsellinic acid) | PKS16 | SAT-KS-AT-ACP ACP-TE | 10 | CytP450, PKS, monooxygenase | grayanic acid | 99%, 73% |
| 5 | Pfur2-2_003072 | I (zearalenone, orsellinic acid) | PKS27 | SAT-KS-AT-PT-ACP-TE | 13 | O-methyltransferase, CytP450, crotonyl-CoA reductase/alcohol dehydrogenase, red-PKS, NRPKS, GATase_7 | grayanic acid | 100%, 38% |
| 6 | Pfur9-3_011125 | IX (mitorubin, atranorin) | PKS23 | SAT-KS-AT-PT-ACP cMT | 11 | alkyl hydroperoxide reductase/Thiol specific, PKS, CytP450, drug resistance transporter | ascochlorin cluster (Acremonium egyptiacum) | 100%, 38% |
| 7 | Pfur3-1_005530 | II (melanins) | PKS13 | SAT-KS-AT-PT-ACP-ACP-TE | 13 | serine/threonine protein kinase, Drug resistance transporter, monooxygenase FAD-binding, NRPKS, short-chain dehydrogenase/reductase SDR, O-methyltransferase, transcription regulator | 100%, 48% | |
| 8 | Pfur69-1_010133 | II (melanins) | PKS14 | SAT-KS-AT-PT-ACP-ACP-TE | 9 | Drug resistance transporter, O-methyltransferase, halogenase, monooxygenase FAD-binding, adh_short (DH-KR), NRPKS, monooxygenase FAD-binding | 6-hydroxymellein (Cladonia uncialis) | 99%, 78% |
Properties of the NR-PKS clusters detected in both the chemotypes of P. furfuracea. NR-PKS = non-reducing PKS. The cluster in bold, in the box (cluster 4 containing Pfur33-1_006185), is the likely cluster for depside/depsidone synthesis. The domain acronyms stand for: KS = keto-synthase, AT = acyltransferase, ACP = acyl carrier protein and KR = ketoreductase. The PKS category is based on the phylogenetic placements of the P. furfuracea NR-PKSs in the PKS groups from Kim et al. [11]. Cluster number refers to Supplementary Table S2 and the PKS number to the antiSMASH cluster, followed by the gene number.