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CLN5-deficient human neurons exhibit reduced lysosomal enzyme activity. (A,B) Microscopic analysis of lysosomal cathepsin B activity in the iPSC-derived human neurons revealed reduced enzyme activity (measured by Magic Red assay—compare pseudo-colour green) in CLN5-deficient neurons (#2 and #3) compared to control neurons. n ≥ 3, * p < 0.05, ** p < 0.01. Scale bar = 20 µm.
