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. 2021 Sep 30;11(10):1437. doi: 10.3390/biom11101437

Figure 1.

Figure 1

Establishment of SupT1 cells stably expressing ankyrin proteins. SupT1 cells were transduced with 1 MOI of VSV-G pseudotyped virus carrying gene encoding the ankyrin protein with EGFP fusion. (A) After 48 h post-transduction, EGFP-positive cells were observed under fluorescent microscopy. Cell imaging was done at 20× magnification with the same exposure time using Axio Observer 7. (B) After cell sorting, the level of ankyrin expression in SupT1 cells was investigated by flow cytometry. EGFP signal indicates ankyrin-expressing SupT1 cells. (C) EGFP intensity in ankyrin-expressing SupT1 cells shown in a bar graph. No ankyrin, AnkA32D3, AnkGAG1D4, and AnkGAG1D4-S45Y represent SupT1 cell control, SupT1 cells expressing Myr (+) AnkA32D3-EGFP, Myr (+) AnkGAG1D4-EGFP, and Myr (+) AnkGAG1D4-S45Y-EGFP, respectively.