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. 2021 Sep 30;11(10):1437. doi: 10.3390/biom11101437

Figure 7.

Figure 7

Sequencing analysis of the HIV-1 N-terminal capsid. WT HIV-1 NL4-3 viral RNA was extracted from culture supernatant harvested from HIV-1-infected cells. Then viral RNA was reverse transcribed into HIV-1 cDNA by RT-PCR. The HIV-1 capsid region was amplified and subjected to sequencing analysis. The diagram shows alignment of HIV-1 capsid sequence against WT HIV-1 NL4-3. Regions of helix 1 (upper) and helix 7 (lower) of HIV-1 capsid indicated in gray. Underlined letters indicate binding sites of ankyrins on the HIV-1 capsid. No ankyrin, AnkA32D3, AnkGAG1D4, and AnkGAG1D4-S45Y represent HIV-1 capsid sequence of viral particles released from HIV-1 infected SupT1 cell controls, SupT1 cells expressing Myr (+) AnkA32D3-EGFP, Myr (+) AnkGAG1D4-EGFP, and Myr (+) AnkGAG1D4-S45Y-EGFP, respectively.