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. 2021 Sep 30;11(10):1437. doi: 10.3390/biom11101437

Figure 9.

Figure 9

HIV-1 MIR viral replication in SupT1 cells expressing binding affinity-enhanced AnkGAG1D4. After HIV-1 challenge, cells were subcultured every 2 days. Cultured supernatant was collected at 5, 9, 13, 17, and 21 days post-infection, then assayed to evaluate HIV-1 production. (A,B) HIV-1 p24 levels were determined using p24 ELISA. † Indicates undetectable level of HIV-1 p24. (C) HIV RNA copies were determined at 13 days post-infection using HIV viral load assay. Data represent mean ± SD from triplicate well. ns, non-significant. * p ≤ 0.05 using unpaired t-test. No ankyrin, AnkA32D3, AnkGAG1D4, and AnkGAG1D4-S45Y represent SupT1 cell control, SupT1 cells expressing Myr (+) AnkA32D3-EGFP, Myr (+) AnkGAG1D4-EGFP, and Myr (+) AnkGAG1D4-S45Y-EGFP, respectively.