Skip to main content
. 2021 Oct 4;9(10):1391. doi: 10.3390/biomedicines9101391

Figure 1.

Figure 1

ECSW therapy protected the rat bladder smooth muscle cells (RBdSMCs) against oxidative stress and mitochondrial damage. (A) Protein expression of NXO-1, * vs. other groups with different symbols (†, ‡), p < 0.001. (B) Protein expression of NOX-2, * vs. other groups with different symbols (†, ‡), p < 0.001. (C) The oxidized protein expression, * vs. other groups with different symbols (†, ‡), p < 0.001 (Note: The left and right lanes shown on the upper panel represent protein molecular weight marker and control oxidized molecular protein standard, respectively). M.W. = molecular weight; DNP = 1–3 dinitrophenylhydrazone. (D) Protein expression of cytosolic cytochrome C (cyt-CytC), * vs. other groups with different symbols (†, ‡), p < 0.001. (E) Protein expression of cyclophilin D (cyc-D), * vs. other groups with different symbols (†, ‡), p < 0.001. (F) Flow cytometric analysis of mean fluorescent intensity (MFI) of DCFDA [i.e., reactive oxygen species (ROS)], * vs. other groups with different symbols (†, ‡), p < 0.001. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n = 6 for each group). Symbols (*, †, ‡) indicate significance (at 0.05 level). ECSW = extracorporeal shock wave; RBdSMCs = rat bladder smooth muscle cells.