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. 2021 Oct 15;9(10):1477. doi: 10.3390/biomedicines9101477

Figure 4.

Figure 4

(A) The schematic shows the location and relationship between ISSag5 and several PI-1 genes, and the PCR results of several genes. (a) PCR results of the ISSag5 (1459 bp) using three antibiotic susceptible and three antibiotic resistant GBS strains by designed primer F and primer R. The ISSag5 sequence is based on searching from NCBI, and documented in all three antibiotic susceptible strains (N96, A28 and P65). (b) PCR results of genes from the beginning of ISSag5 to the end of PI-1 class C sortase (8571 bp) by designed primer F and PI-1 primer R. (c) PCR results of PI-1, PI-2a and PI-2b genes in antibiotic-susceptible and antibiotic-resistant III/ST-17 GBS strains. (B) The schematic shows the beginning of ICESag37 from rumA to the end of ICESag37 at integratase. The primers were designed for ICESag37 head and ICESag37 tail, respectively. The multiplex PCR was performed in three antibiotic-resistant and three antibiotic-susceptible III/ST-17 GBS isolates for verification.