Table 1.

Principal oligonucleotide primers used in this study.

Gene	Sequence (5′−3′)	Product Size (bp)	Reference (DOI)
ISSag5	F: CAACAGATGCATCTCATTCTAATC	1459	this study
	R: TTCCTGCACATCTCAACTAA
PI-1	F: CAAGATTGACCGGGTGGAGA	325	10.1016/j.micpath.2018.01.035 [30]
	R: ATGGGCAGTTAGAACGGCAT
PI-2a	F: CGGGGTGCAAGTCAATAAGG	264	10.1016/j.micpath.2018.01.035 [30]
	R: GGAGCAGGGCATTTAGAAGGT
PI-2b	F: CTCTGCTACCACCAAAGCGT	665	10.1016/j.micpath.2018.01.035 [30]
	R: GTGGGGGTAGGCTTAATGGC
ICESag37 head	F: ACATAGCCCCGTCAGTATG	816	this study
	R: ATCACGTGGAGTGGTAGTG
ICESag37 tail	F: GCAACGTGGTGAATTGATAGGG	1011	10.3389/fmicb.201.7.01921 [32]
	R: AAAACTGCACGATCAAACTCCG

ICE: integrative and conjugative element.