FIG. 3.

Binding of full-length CBP to mutants of GST-SREBP1 (a) and GST-CREB (b). GST-CREB(1-290) phos. refers to GST fusion protein containing the CREB activation domain phosphorylated by PKA. Other mutations in panel b refer to substitutions of CREB residues with residues from SREBP. Only the DIEDML substitution conferred phosphorylation-independent binding. The schematic depicts substitution of CREB sequences into SREBP. Dots refer to SREBP residues left unchanged. Percent binding is indicated on the right. NE, nuclear extract.