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. 2021 Oct 9;13(20):5039. doi: 10.3390/cancers13205039

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Ide promotes an early memory subset of DNTs that have better proliferative capacity. (A) Representative flow plots showing memory status. Memory status of ctrl-DNT and Ide-DNT on day 17 of expansion determined by CD45RA and CD62L expression. T_naive/SCM = CD45RA⁺ CD62L⁺; T_CM = CD45RA⁻ CD62L⁺; T_EM = CD45RA⁻ CD62L⁻; T_effector = CD45RA⁺ CD62L⁻. (B) Frequency of DNTs in early memory subset T_CM/SCM (CD45RA⁺/CD62L⁺ and CD45RA⁻ CD62L⁺; left), T_EM (CD45RA⁻ CD62L⁻; middle), and T_EFF (CD45RA⁺ CD62L⁻; right) from day 7 to day 17 of ex vivo DNT expansion culture. (C) T_CM/SCM cell number of ctrl-DNT and Ide-DNT on day 17 of ex vivo DNT expansion culture. Each dot represents DNTs from one donor. The cell number is normalized to day 0 DNT number. (D) Relative expression of memory associated genes, TCF7, LEF1, SELL, and IL7R, in ctrl-DNT and Ide-DNT determined by qPCR. The expression shown is relative to the expression of housekeeping gene, HPRT. Each of the paired symbols represent DNTs from one donor. (E) Expansion of ctrl-DNT (black) and Ide-DNTs (red) in the presence of DNT-susceptible AML cell line, OCI-AML3. Expansion fold relative to the number of DNTs at the start of co-culture was determined after 2 days and 8 days (Left). Absolute number of viable DNTs from each treatment group after co-culture with OCI-AML3 (Right). The results shown are representative of three independent experiments. (F) Memory status of DNTs 8 days post co-culture with OCI-AML3 determined by flow cytometry. The result shown is representative of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.

Ide promotes an early memory subset of DNTs that have better proliferative capacity. (A) Representative flow plots showing memory status. Memory status of ctrl-DNT and Ide-DNT on day 17 of expansion determined by CD45RA and CD62L expression. T_naive/SCM = CD45RA⁺ CD62L⁺; T_CM = CD45RA⁻ CD62L⁺; T_EM = CD45RA⁻ CD62L⁻; T_effector = CD45RA⁺ CD62L⁻. (B) Frequency of DNTs in early memory subset T_CM/SCM (CD45RA⁺/CD62L⁺ and CD45RA⁻ CD62L⁺; left), T_EM (CD45RA⁻ CD62L⁻; middle), and T_EFF (CD45RA⁺ CD62L⁻; right) from day 7 to day 17 of ex vivo DNT expansion culture. (C) T_CM/SCM cell number of ctrl-DNT and Ide-DNT on day 17 of ex vivo DNT expansion culture. Each dot represents DNTs from one donor. The cell number is normalized to day 0 DNT number. (D) Relative expression of memory associated genes, TCF7, LEF1, SELL, and IL7R, in ctrl-DNT and Ide-DNT determined by qPCR. The expression shown is relative to the expression of housekeeping gene, HPRT. Each of the paired symbols represent DNTs from one donor. (E) Expansion of ctrl-DNT (black) and Ide-DNTs (red) in the presence of DNT-susceptible AML cell line, OCI-AML3. Expansion fold relative to the number of DNTs at the start of co-culture was determined after 2 days and 8 days (Left). Absolute number of viable DNTs from each treatment group after co-culture with OCI-AML3 (Right). The results shown are representative of three independent experiments. (F) Memory status of DNTs 8 days post co-culture with OCI-AML3 determined by flow cytometry. The result shown is representative of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.