Table 2.
IHC/IF staining conditions for antibodies used in this study.
| Antibody | Species | Company | Catalogue No. | Condition | Dilution |
|---|---|---|---|---|---|
| CA19-9 | mouse | Thermo | MA5-12421 | ST Citrate | 1:500 |
| CLDN1 | rabbit | Abcam | ab15098 | ST Tris | 1:100 |
| cl-CASP3 | rabbit | Cell Signaling | 9664 | ST Citrate | 1:1000 |
| E-CAD | mouse | BD Bioscience | 610182 | ST Citrate | 1:1000 |
| HA-tag | rabbit | Cell Signaling | 3724 | ST Citrate | 1:500 |
| Ki-67 | mouse | Dako | M7240 | ST Citrate | 1:200 |
| Ki-67 | rabbit | Invitrogen | MA5-14520 | ST Citrate | 1:100 |
| KRT19 (IF) | mouse | Dako | M0888 | ST Citrate | 1:100 |
| KRT19 (IHC) | mouse | Dako | M0888 | Pronase | 1:100 |
| KRT7 | mouse | Dako | M7018 | Pronase | 1:200 |
| mCherry | rabbit | Abcam | ab167453 | No AGR | 1:500 |
| MUC5AC | mouse | Santa Cruz | sc-33667 | ST Citrate | 1:100 |
| N-CAD (IF) | rabbit | Cell Signaling | 13116 | ST Citrate | 1:100 |
| N-CAD (IHC) | rabbit | Cell Signaling | 13116 | ST Tris | 1:100 |
| P14 | mouse | Cell Signaling | 2407S | ST Citrate | 1:50 |
| P16 | rabbit | Abcam | ab108349 | ST Citrate | 1:400 |
| P21 | rabbit | Abcam | ab109520 | ST Citrate | 1:300 |
| P53 | mouse | Santa Cruz | sc-47698 | ST Citrate | 1:100 |
| pRB | rabbit | Cell Signaling | 8516 | ST Citrate | 1:200 |
| RB | mouse | Cell Signaling | 9309 | ST Citrate | 1:400 |
| VIM | rabbit | Cell Signaling | 5741S | ST Citrate | 1:500 |
| ZO-1 | mouse | Thermo | 33-9100 | ST Citrate | 1:500 |
| γH2AX | rabbit | Cell Signaling | 9718 | ST Citrate | 1:400 |
AGR, antigen retrieval; ST, steamer.