Table 6.
Detection of the HCMV genome in GBM tissue specimens using PCR methods.
|
HCMV Genes |
No Def | Nested | RT, Taq, q | RT | ddPCR | Samples | % | %/tot 2676 | Analysis |
|---|---|---|---|---|---|---|---|---|---|
| Pos/tot | Pos/tot | Pos/tot | Pos/tot | Pos/tot | Pos/tot | ||||
| No def | 13/22 | 31/146 | 106/441 | 0/45 | 150/654 | 22.9 | 24.4 | 19 | |
| gB | 5/13 | 84/258 | 94/366 | 183/637 | 28.7 | 23.8 | 19 | ||
| pp71 | 5/5 | 22/25 | 27/30 | 90 | 1.1 | 4 | |||
| IE1 | 26/73 | 21/162 | 25/25 | 72/260 | 27.7 | 9.7 | 12 | ||
| pp65 | 6/18 | 10/11 | 169/612 | 185/641 | 28.9 | 24.0 | 9 | ||
| US28 | 8/8 | 8/8 | 18/44 | 34/60 | 56.7 | 2.2 | 3 | ||
| UL144 | 4/5 | 0/116 | 4/5 | 8/126 | 6.3 | 4.7 | 3 | ||
| Others | 49/50 | 65/198 | 5/20 | 119/268 | 44.4 | 10.0 | 16 | ||
| Samples | 90/121 | 151/604 | 489/1817 | 48/89 | 0/45 | 778/2676 | 29.1 | ||
| % | 74.4 | 25 | 26.9 | 53.9 | 0 | 29.1 | |||
| %/tot 2676 | 4.5 | 22.6 | 67.9 | 3.3 | 1.7 | 100 | |||
| Analysis | 14 | 20 | 47 | 3 | 1 | 85 |
PCR: polymerase chain reaction; RT: reverse transcriptase; qPCR: quantitative PCR; Taq: TaqMan PCR; ddPCR: droplet digital PCR; gB: glycoprotein B; PP: polypeptide; US: unique short; UL: unique long; tot: total; %: percent; No def: not defined. Other primers to HCMV include UL17, UL27, UL69, UL96, US2, US11, US17, UL112, and UL73.