Figure 3.

Optimization of experimental conditions for hydrogen peroxide detection, performed by analyzing 10 µL of a 100 µmol L⁻¹ hydrogen peroxide standard solution (the selected conditions are highlighted). (a) CL signals obtained in origami µPADs containing different amounts of luminol and potassium hexacyanoferrate(III); (b) CL signals obtained with carbonate transport buffers at different pH; (c) CL signals obtained using different carbonate transport buffer volumes. In each measurement, the optimal values for the other experimental conditions were used. Each value is the mean ± SD of six measures performed in three origami µPADs.