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tt15 seeds remain buoyant because they do not release mucilage. (a) Seed buoyancy was examined for 200 seeds of tt15 or ugt80A2-3 mutants in Ws-4 or Col-0 accessions by comparing to wild-type seeds after imbibition in water for 10 min. (b) direct ruthenium red staining of 100 seeds per genotype. Bars = 1 mm. Wild-type, WT, tt15-2 ugt80A2-3, tt15-2 A2. Similar results were obtained with seed lots from 3 biological replicates/genotype.
