Table 1.
Media composition and layout of the differentiation protocol to generate hPSC-CMC, according to [21] with modifications.
| Day | Basal Medium | Supplements |
|---|---|---|
| 0 | RPMI1640 B27 without insulin 50 μg/mL l-ascorbic acid 50 µg/mL penicillin and 5 µg/mL streptomycin |
CHIR99021 BMP4 |
| 1 | RPMI1640 B27 without insulin 50 μg/mL l-ascorbic acid 50 µg/mL penicillin and 5 µg/mL streptomycin |
CHIR99021 |
| 2 | RPMI1640 B27 without insulin 50 μg/mL l-ascorbic acid 50 µg/mL penicillin and 5 µg/mL streptomycin |
- |
| 3–6 | RPMI1640 B27 without insulin 50 μg/mL l-ascorbic acid 50 µg/mL penicillin and 5 µg/mL streptomycin |
10 μM XAV939 |
| 7 | RPMI1640 B27 without insulin 50 μg/mL l-ascorbic acid 50 µg/mL penicillin and 5 µg/mL streptomycin |
- |
| 8–13 | RPMI 1640 without glucose 50 µg/mL penicillin and 5 µg/mL streptomycin |
4 mM sodium l-lactate |
| 14–18 | RPMI1640 B27 with insulin 50 μg/mL l-ascorbic acid 50 µg/mL penicillin and 5 µg/mL streptomycin |
- |
| 19–300 | RPMI1640 B27 without insulin 50 μg/mL l-ascorbic acid 50 µg/mL penicillin and 5 µg/mL streptomycin |
- |