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. 2021 Oct 2;10(10):2640. doi: 10.3390/cells10102640

Figure 3.

Figure 3

BMP-7 attenuates the expression of pyroptotic proteins caspase-1, IL-1β, and IL-18 in the diabetic heart. (A–C) Representative photomicrographs of heart sections stained in control group (a–e), STZ group (f–j) and STZ-BMP7 group (k–o) with sarcomeric-α actin (green, stained cardiac myocyte) (b, g, l) and pyroptotic markers caspase-1 (A), IL-1β (B) and IL-18 (C) in red (a, f, k) with nuclei stained (c, h, m) by DAPI in blue, merged (d, i, n) and enlarged (e, j, o) image. (D,F,H) Quantitative analysis-derived histograms of pryoptotic caspase-1 (D), IL-1β (F), and IL-18 (H) +ve cardiomyocytes (colocalization of both red and blue DAPI in green cardiomyocyte) were quantified over total DAPI in heart sections of mice. (E,G,I) Representative Western blots and densitometric analysis of caspase-1 (E), IL-1β (G), and IL-18 (I) markers are shown. Quantities are presented as an arbitrary unit (A.U). Statistical analysis was performed using One-Way ANOVA, which was followed by the Tukey test. Error bars= mean ± S.E.M. * p < 0.05, ** p < 0.01, *** p < 0.001; Scale bar = 100 µm; n = 16/group.