Figure 1.
Power analysis plots with power as a function of the co-efficient of variation (CV), using (A): 27 and (B): 32 replicates respectively to detect FC values ranging from 2.0 to 3.0. Type I error rate is conservatively fixed at α = 2.5 × 10−6 (α = 0.05 after Bonferroni Correction). (C) MA Plot examining differential gene expression between EA and AA men. The plot visualizes the differences between measurements taken in two samples, by transforming the data onto M (log ratio) and A (mean average) scale. M (Y-axis) is plots differential gene expression (log2 (EA/AA). A (X-axis) plots transcript abundance (0.5 log2 AA + 0.5 log2 EA). significant transcripts (q < 0.1) are highlighted in red. (D) Volcano plot depicting the DE genes in the contrast between AA and EA men. The horizontal axis is the log fold change, and the vertical axis is the negative base-10 logarithm of the adj p-value or q-value. The red-dotted lines represent the threshold. The up-regulated genes (positive log fold change) are shown in red, while the down-regulated genes are blue. AA: African–American; EA: European–American.