Figure 3.

Post-translational modification sites for human (top) and mouse (bottom) GRα pertaining to phosphorylation, ubiquitination and sumoylation. The human GRα protein consists of 777 and the mouse GRα of 784 amino-acid residues, with both of these proteins undergoing PTMs at various sites. Phosphorylation (light blue) occurs primarily at serine residues while, sumoylation (yellow) and ubiquitination (purple) occur at lysine residues for both receptors. Moreover, phosphorylation at S404 in humans and S412 in mice (dark blue), as well as ubiquitination of K419 in humans and K426 in mice, is known to modulate receptor levels. The precise site of symolation which affect GRα stability, has not yet been determined. Indicated in red are the alanine to threonine point mutations which yield the dimerization deficient GR mutant, GRdim.