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Search for transcription products from either strand of the DHFR and CK18 genes. Total RNA from NT2 or hNT cells was reverse transcribed with primers specific for the DHFR or CK18 mRNA or for putative antisense RNA generated by transcription of the nontranscribed strand of these genes. As a control, RT reactions were performed with no primer. The resulting cDNA was then measured by semiquantitative PCR.
