Table 2.
Secondary antibodies and other reagents.
| Antibodies and Other Reagents | Source | Dilution | Label |
|---|---|---|---|
| Goat anti-mouse IgG Ab (#115-165-166) | Jackson ImmunoResearch, Ely, United Kingdom | 1/200 | Cy3 |
| Goat anti-rabbit IgG Ab (#A-11034) | Invitrogen, Darmstadt, Germany |
1/200 | Cy3 |
| Goat anti-mouse IgG Ab (#A-11029) | Invitrogen, Darmstadt, Germany | 1/200 | Alexa Fluor 488 |
| Goat anti-rabbit IgG Ab (#A-11034) | Invitrogen, Darmstadt, Germany |
1/200 | Alexa Fluor 488 |
| CC2 solution (#950-223) | Ventana Medical Systems, Lund Sweden |
Ready-to-use | w/o |
| VECTASHIELD mounting medium (#H-1000) |
Vector Laboratories, Burlingame, CA, USA |
Ready-to-use | w/o |
| TSA plus fluorescein (#NEL741E001K) | PerkinElmer, Rodgau, Germany |
1/200 | Fluorescein |
| TSA plus cyanine 3 (#NEL744E001KT) |
PerkinElmer, Rodgau, Germany |
1/200 | Cyanine 3 |