Table 3.
Efficiency of immunomorphological detection of CD38 in MCs.
| Organ | Primary Antibodies to CD38 (SP149) Rabbit Monoclonal Antibody (Cell Marque, USA) | Primary Murine Antibodies to CD38 (Kindly Provided by Fabio Malavasi, University of Torino, Italy) | ||||||
|---|---|---|---|---|---|---|---|---|
| Number CD38+ MC (%, M ± m) | Expression Intensity (%) | Number CD38+ MC (%, M ± m) |
Expression Intensity (%) | |||||
| + (M ± m) |
++ (M ± m) |
+++ (M ± m) |
+ (M ± m) |
++ (M ± m) |
+++ (M ± m) |
|||
| Skin (normal; n = 5) | 5.2 ± 0.5 | 65.2 ± 3.1 | 22.3 ± 1.9 | 12.5 ± 1.1 | 8.8 ± 0.5 | 52.4 ± 4.2 | 39.2 ± 2.5 | 8.4 ± 0.5 |
| Skin (neurotrophic ulcers; n = 6) | 7.3 ± 0.6 ∆ | 13.2 ± 0.8 ∆ | 62 ± 4.3 ∆ | 24.8 ± 1.6 ∆ | 14.1 ± 1.2 ∆ | 15.6 ± 1.1 ∆ | 66.7 ± 4.3 ∆ | 17.7 ± 1.3 ∆ |
| Breast (normal; n = 5) | 3.2 ± 0.2 | 58.9 ± 5.1 | 32.7 ± 2.8 | 8.4 ± 0.9 | 8.4 ± 0.6 | 45.6 ± 3.4 | 47.1 ± 3.1 | 7.3 ± 0.5 |
| Mammary cancer (n = 6) | 3.1 ± 0.3 | 23.5 ± 1.9 ∆ | 61.3 ± 5.3 ∆ | 15.2 ± 1.4 ∆ | 12.3 ± 1.3 ∆ | 35.6 ± 2.2 ∆ | 55.6 ± 3.2 ∆ | 8.8 ± 0.4 |
| Bone marrow (mastocytosis; n = 4) | * | - | - | - | 8.2 ± 0.6 | 65.2 ± 4.3 | 29.5 ± 1.8 | 5.3 ± 0.3 |
| Tonsilla (recurrent tonsillitis; n = 10) | * | - | - | - | 9.7 ± 0.5 | 59.4 ± 4.1 | 35.9 ± 2.2 | 4.7 ± 0.4 |
Legend: *, single cells; +, weak expression; ++, moderate expression; +++, pronounced expression; ∆, p < 0.05.