Figure 8.

rFlaA:Betv1 induces a transcriptional shift towards HIF-1a-mediated glycolytic metabolism in BMDMs. C57BL/6 BMDMs were differentiated from mouse bone marrow and stimulated with either LPS as a positive control or equimolar amounts of either rFlaA + rBet v 1 or rFlaA:Betv1 for 2 to 48 h (A). Cells were harvested and used for either RNA-seq (B,C) or Western blot analyses (D). To characterize the transcriptional status of the different samples, principal component analysis (B) and gene set enrichment analysis of the most significantly regulated KEGG pathways (C) was performed. BMDMs stimulated for 2, 6, 24, or 48 h with either LPS, rFlaA + rBet v 1, or rFlaA:Betv1 were analyzed by Western blot for expression levels of the indicated molecules (D). Data are either mean (C) or representative (D) results of three to four independent experiments using either one RNA preparation (B,C) or one lysate (D) per experiment.