Figure 5.
Effect of resveratrol on the basal cytosolic and mitochondrial Ca2+ levels in control and parkin-mutant fibroblasts. Spectrofluorometric measurements of cytosolic (a) and mitochondrial Ca2+ (b) levels in control (open bar) and parkin-mutant (black bar) fibroblasts loaded, respectively, with Fluo-4AM and X-Rhod-1AM, exposed to either vehicle (DMSO) or 25 μM resveratrol for 30 min. The values, expressed as nM, represent the means ± SEM from 3 independent experiments under each condition. The significance was determined by two-way ANOVA with Tukey post hoc; *, p ˂ 0.01; **, p ˂ 0.001; ****, p ˂ 0.0001. For more details, see Materials and Methods.