Figure 8.

Effect of resveratrol on the basal cellular Ca²⁺ level in control and parkin-mutant fibroblasts in the presence of dantrolene (Dan). Spectrofluorometric measurements of cytosolic (a) and mitochondrial (b) Ca²⁺ levels in control (open bar), and parkin-mutant (black bar) fibroblasts loaded, respectively, with Fluo-4AM and X-Rhod-1AM. The cells were exposed for 30 min to vehicle (DMSO) or 25 μM resveratrol (RSV). Where indicated, the cells were incubated for 30 min with 10 µM dantrolene (Dan) alone or co-incubated with resveratrol (RSV+Dan). The values, expressed as nM, represent the means ± SEM from 3 independent experiments under each condition. The significance was determined by two-way ANOVA with Tukey post hoc; *, p ˂ 0.01; **, p ˂ 0.001; ****, p ˂ 0.0001. The statistical significance of Ca²⁺ level among DMSO and RSV treatments is presented in Figure 5 and omitted herein to streamline the figure. For more details, see Materials and Methods.