Table 1.
FEVER Probe | FEVER Probe Sequence | RNA Target Sequences |
---|---|---|
IAV | 5′-/5Alexa532N/CGCGATGAGGAGTGCCTGATTAATGATCCCTGGGTTTA/BiodT/CGCG/3BHQ-1/-3′ | Match: UAAGCAAAACCCAGGGAUCAUUAAUCAGGCACUCCUCAAUUGC (13,710.3 g/mol) |
Mismatch: UAAGCAAAACCCAGGGAUCGUUAAUCAGGCACUCCUCAAUUGC (13,726.3 g/mol) | ||
IBV | 5′-/5Alexa532N/CGCGATGAGGGAATGCCAAGAACCATAGCATGGATGGA/BiodT/CGCG/3BHQ-1/-3′ | Match: UUUGGACCAUCCAUGCUAUGGUUCUUGGCAUUCCCUCAAUUAC (13,549 g/mol) |
Mismatch: UUUGGACCAUCCAUGCUAUGUUUCUUGGCAUUCCCUCAAUUAC (13,510 g/mol) |
Stem region of probes is underlined. The single mismatch base of the mismatch RNA target sequences is bold.