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. 2021 Sep 30;11(10):367. doi: 10.3390/bios11100367

Table 1.

Sequences of FEVER influenza MB probes and the synthetic RNA match and single base pair mismatch target sequences.

FEVER Probe FEVER Probe Sequence RNA Target Sequences
IAV 5′-/5Alexa532N/CGCGATGAGGAGTGCCTGATTAATGATCCCTGGGTTTA/BiodT/CGCG/3BHQ-1/-3′ Match: UAAGCAAAACCCAGGGAUCAUUAAUCAGGCACUCCUCAAUUGC (13,710.3 g/mol)
Mismatch: UAAGCAAAACCCAGGGAUCGUUAAUCAGGCACUCCUCAAUUGC (13,726.3 g/mol)
IBV 5′-/5Alexa532N/CGCGATGAGGGAATGCCAAGAACCATAGCATGGATGGA/BiodT/CGCG/3BHQ-1/-3′ Match: UUUGGACCAUCCAUGCUAUGGUUCUUGGCAUUCCCUCAAUUAC (13,549 g/mol)
Mismatch: UUUGGACCAUCCAUGCUAUGUUUCUUGGCAUUCCCUCAAUUAC (13,510 g/mol)

Stem region of probes is underlined. The single mismatch base of the mismatch RNA target sequences is bold.