FIG. 10.

Nuclear import of IκBα is not inhibited by a dominant-negative Ran protein. Nuclear import reactions with the indicated substrate proteins were performed in the absence (A, B, and E to H) or presence (C and D) of 10 nM leptomycin B (LMB). Parallel import reactions were performed in the absence (A, C, E, and G) or presence (B, D, F, and H) of 5 μg of the RanQ69L protein preloaded with GTP. Localization of the import substrates was determined by indirect immunofluorescence with a monoclonal antibody directed against GST.