Table 4.
MRD monitoring in two AML M3v cases. The level of PML-RARA and NPM1-RARA transcripts were normalized to the reference gene ABL1 as the normalized copy number (based on the ∆Cp method).
| Treatment Day | Day 1 | Day 1 | Day 1 | Day 25 | Day 25 | Day 25 | Day 42 | Day 88 
|
Day 88 | Day 112 | Day 140 |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Method of MRD detection | BM FC | FISH | qPCR | BM FC | FISH | qPCR | BM FC | FISH | qPCR | qPCR | qPCR |
| Patient 1 | 82% | 93% | 404% | 49% | 46% | 28% | 2% | 0.01% | neg | ||
| Patient 2 | 77% | 89% | 32.6% | 0.06% | 0.04% | 0.005% | neg | neg | neg |
: start of arsenic-trioxide treatment. Abbreviations: BM FC: bone marrow flow cytometry; FISH: fluorescent in situ hybri-dization; MRD: minimal residual disease; qPCR: quantitative polymerase chain reaction.