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Structure of DNA constructs for luciferase reporter assay. Cloning of the HPSE gene fragment of intron 9 was performed using PCR II-TOPO vector. The insulator fragments were digested with Hind III and Xho I with subsequent ligation into phosphatase-treated pGL4.26 (luc2/minP/Hygro) vector. Three sense and three antisense DNA constructs were prepared. The constructs were designated with Roman numerals I, II, and III, and represent possible allelic variants in the general population.
