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. 2021 Sep 29;10(10):2586. doi: 10.3390/cells10102586

Figure 3.

Figure 3

To examine the impact of chemical induction of MPT on caspase-3 activation, single isolated myofibers were treated with either 300 nM Bz423, 300 nM BZ423 + 20 µM Ac-ATS010-KE (to inhibit caspase-3), or 300 nM BZ423 + 1 µM TR002 (to inhibit MPT) for 24 h, and caspase-3 FLICA signal was determined at baseline and post-treatment. (A) Representative brightfield and fluorescence images of single FDB muscle fibers following 24 h treatment with Bz423. Scale bars represent 100 µm. (B) While TR002 prevented an increase in caspase-3 FLICA fluorescence over the 24 h treatment period, Ac-ATS010-KE decreased signal compared to Day 0. Treatment with BZ423 induced an increase in caspase-3 FLICA, indicating an increase in caspase-3 activity following treatment. Biological replicates are identified by data points of different colors within a given treatment, while technical replicates share the same color data point. n = 2 biological replicates were used for pre-treatment and 300 nM Bz423-treated myofibers, while n = 1 biological replicate was used for all other groups. **** p < 0.0001 versus Day 0.