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. 2021 Sep 23;10(10):2520. doi: 10.3390/cells10102520

Figure 1.

Figure 1

Figure 1

Apoptosis induction by hydroxyurea plus entinostat. PDAC cell lines were treated with various concentrations of entinostat (MS-275) and/or hydroxyurea (HU) for 24 h and 48 h. Cell cycle distribution and cell death were measured by flow cytometry of PI-stained cells. (A) S821, (B) 8296, (C) 8248, and (D) S411 cells were incubated for 24 h and 48 h with 1 µM (+), 5 µM entinostat (++), and/or 1 mM hydroxyurea (+). Data are mean ± SD values (24 h: n = 3; 48 h: n = 4). (E–H) The cells were treated with 1 µM entinostat (+) or 5 µM entinostat (++) plus 1 mM hydroxyurea (+) for 48 h and apoptosis was determined by flow cytometry for annexin-V/PI (n = 3). Data were statistically analyzed using one-way ANOVA (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).